ASCP Histotechnician Certification 2025 – 400 Free Practice Questions to Pass the Exam

Question: 1 / 400

What effect does increasing the thickness of tissue sections have on microscopic examination?

It allows for better staining of tissues

It can obscure features and make diagnosis more challenging

Increasing the thickness of tissue sections can indeed obscure features and make diagnosis more challenging. Thicker sections can result in overlapping cell layers and reduced clarity, making it difficult to discern individual cellular elements, structures, and tissue architecture under the microscope. As a result, pathologists may struggle to make accurate assessments or diagnoses due to the compromised visibility of important histological details.

Furthermore, the staining process may be less effective in thicker sections because the staining reagents may not penetrate uniformly, leading to uneven coloration and difficulties in interpreting the staining patterns that are critical for diagnosis. Properly prepared, thinner sections usually offer superior microscopic resolution, providing a clearer view of the cellular organization and allowing for more precise evaluations.

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It improves the visibility of cellular details

It accelerates the observation process

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