ASCP Histotechnician Certification 2025 – 400 Free Practice Questions to Pass the Exam

Hematoxylin and eosin (H&E) stain is widely utilized in histology because it effectively highlights the structure and cellular components within tissue sections. Hematoxylin stains nuclei blue or purple, allowing for clear visualization of nuclear detail, while eosin stains the cytoplasm and extracellular matrix in varying shades of pink. This dual staining technique provides a comprehensive overview of the tissue architecture, making it invaluable in diagnostics.

The other staining options serve different purposes: Giemsa stain is primarily employed in the examination of blood smears and cytological samples, focusing on nucleic acids and certain cellular inclusions; Gram stain differentiates bacteria based on their cell wall properties; and trichrome stain is typically used to identify connective tissue components and highlight fibrosis in tissue sections. While each of these stains is significant in its own right, H&E remains the cornerstone of histopathological examination due to its broad applicability and the clarity it provides in assessing tissue morphology.